Recommended Applications: WB, IHC, ICC, FC
Species Reactivity: Human, Mouse, Rat

Mol. Weight: 60 kDa
UniProt ID: P04040

Description: Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1. 5 A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erythrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflammation) (2).

Specificity: A synthetic peptide corresponding to residues near the C-terminus of human Catalase was used as an immunogen.

Recommended Dilutions:
WB: 1:10,000 20,000
IHC: 1:100 250
ICC: 1:100 250
Flow Cytometry: 1:50

A. Western blot analysis on Hela cell lysate using anti-Catalase RabMAb (cat. # 2058-1), dilution 1:20,000.	B. Immunohistochemical staining of paraffin-embedded human normal brain tissue using anti-Catalase RabMAb (cat. #2058-1).

*Determined species reactivity – for other species not listed cross reactivity may be possible but not tested.